Journal: Translational Oncology

Article Title: Global Conservation of Protein Status between Cell Lines and Xenografts 1

doi: 10.1016/j.tranon.2016.05.005

Figure Lengend Snippet: Comparison of In Vitro and In Vivo Data for Each Model

Article Snippet: SNB19 glioma cell line was given by N. Auger (Institut Curie, Paris, France).

Techniques: Comparison, In Vitro, In Vivo

Journal: Cancer Management and Research

Article Title: The Potential Significance of the EMILIN3 Gene in Augmenting the Aggressiveness of Low-Grade Gliomas is Noteworthy

doi: 10.2147/CMAR.S463694

Figure Lengend Snippet: EMILIN3 gene function experiment in LN229. qRT-PCR transfected LN229 human glioma cells efficiently (A) . The CCK-8 experiment and live/dead staining findings demonstrated that overexpression of the EMILIN3 gene may increase LN229 cell line proliferation and vitality ( B and C ). Overexpression of the EMILIN3 gene enhances the migration, invasiveness, and colony formation of the LN229 cell line (D-F ).

Article Snippet: The LN229 and HS-683 human glioma cell line were purchased from iCell Bioscience Inc, and grown in DMEM media (Gibco, USA) with 10% foetal bovine serum (BI, USA) and 1g/mL penicillin/streptomycin (Hyclone, USA) at 37 degrees Celsius and 5% carbon dioxide.

Techniques: Quantitative RT-PCR, Transfection, CCK-8 Assay, Staining, Over Expression, Migration

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: GBM cell viability in the dose-dependent manner: (a) Curcumin inhibited GBM cell viability in a dose-dependent manner. Cell viability was evaluated by MTT assay for LN18 and LN428 cells treated with the indicated doses of curcumin. Values are the means ± SD from three experiments; * P < 0.05, * * P < 0.01, * * * P < 0.001; (b) MTT assays of LN18 and LN428 cells treated with 5 μM curcumin and irradiated with γ-rays and neutrons. Values are the means ± SD from three experiments; * P < 0.05, * * * P < 0.001; (c) 3D spheroid growth assay of LN18 and LN428 cells treated with curcumin and radiation for four days. Phase-contrast images indicated that untreated cells formed polarized spheroids, but cells exposed to curcumin and radiation did not. Cells exposed to γ-rays and neutron radiation (5 Gy, 5 GyE).

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: MTT Assay, Irradiation, Growth Assay

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: The radiosensitizing effects of curcumin on GBM cells: (a) Radiosensitivity of LN18 and LN428 cell lines with and without curcumin (5 μM) after various doses of γ-ray and neutron radiation was measured by colony-forming assay. The x-axis shows the equivalent dose, expressed as GyE (Gray equivalent). Values are the means ± SD from three experiments.

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques:

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Fitting parameters α and β for survival curves in cell

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques:

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Radiation dose required for 50% cell death and radiosensitivity enhancement factor (REF)

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques:

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Effects of curcumin and radiation on apoptosis and autophagic cell death in GBM cells: (a) LN18 and LN428 cells were treated with neutron and/or curcumin for 24 h. Cell cycle distribution (subG1) was analyzed quantitatively by flow cytometry. Values are the means ± SD from three experiments; * P < 0.05, * * P < 0.01, * * * P < 0.001; (b) LN18 and LN428 cells were exposed to curcumin (10 μM) and/or 5 Gy γ-ray or neutron radiation for 48 h for Annexin V staining. Values represent means of three experiments ± SD; * P < 0.05, * * P < 0.01, * * * P < 0.001; (c) Analysis of cell death in two GBM cell lines 72 h after treatment with curcumin plus radiation using a cell death detection kit. Values are the means ± SD from three experiments; * P < 0.05, * * * P < 0.001. (D, E) TUNEL staining and cyto-ID staining of LN18 and LN428 cells with and without neutron beam or with and without curcumin treatment.

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Flow Cytometry, Staining, TUNEL Assay

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of apoptotic cells by cell cycle detection on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Control

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of apoptotic cells by annexin V/PI staining on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Staining, Control

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of apoptotic cells by cell death kit on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Control

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Effects of curcumin and neutron on ROS in GBM cells: (a) Cell lysates (30 μg) were immunoblotted (IB) with indicated antibodies; (b, c) Analysis of ROS generation in LN18 and LN428 cell line 24 h after treatment with curcumin, neutron or combination by a caspase 3 assay kit and ROS detection kit. Values are the means ± SD from three experiments; * P < 0.05, * * P < 0.01, * * * P < 0.001.

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Caspase-3 Assay

Journal: Journal of Radiation Research

Article Title: Interaction of curcumin with glioblastoma cells via high and low linear energy transfer radiation therapy inducing radiosensitization effects

doi: 10.1093/jrr/rrac016

Figure Lengend Snippet: Detection of ROS on LN18 and LN428

Article Snippet: Human glioma LN428 cells were obtained from the KCLB and were grown in RPMI 1640 medium supplemented with 10% FBS, glutamine, HEPES and antibiotics at 37°C in a 5% CO2 humidified incubator.

Techniques: Control